《植物生理学报》 2009, 45(6): 537-543

超量表达IPT 和KN1 基因对转基因烟草生长发育的影响

胥珊, 孙一铭, 贾之春, 罗克明*

西南大学生命科学学院, 三峡库区生态环境教育部重点实验室, 重庆400715

通信作者：罗克明；E-mail: luokeming@hotmail.com；Tel: 023-68254530

摘　要：

为了比较异戊烯转移酶基因IPT和玉米homeobox基因KN1超量表达对植物生长发育的影响, 将35S::IPT和35S::KN1 分别导入烟草。观察发现, 过量表达 IPT 和 KN1 基因对植株再生频率、形态、生长周期和顶端优势等方面的影响均相似, 但在生根和开花结籽方面, 35S::IPT 转基因植物所受影响更显著。细胞分裂素含量检测表明, 35S::IPT 转基因植物叶片中 细胞分裂素的含量高于35S::KN1 转基因植物。

关键词：IPT 基因; KN1 基因; CaMV 35S; 转基因烟草

收稿：2008-12-05   修定：2009-04-23

资助：国家自然科学基金项目(30871576)、人事部留学回国人员科技活动择优资助项目和重庆市自然科学基金重点项目(CSTC, 2009BA1004)。

Effects of Overexpression of IPT and KN1 on Development and Growth in Transgenic Tobacco Plants

XU Shan, SUN Yi-Ming, JIA Zhi-Chun, LUO Ke-Ming*

Key Laboratory of Eco-Environments of Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China

Corresponding author: LUO Ke-Ming; E-mail: luokeming@hotmail.com; Tel: 023-68254530

Abstract:

To compare the effects of overexpression of isopentenyl transferase gene (IPT) and Knotted1 gene (KN1) on plant development and growth, IPT and KN1 under the control of the 35S promoter were introduced into Nicotiana tabacum cv. xanthi, respectively. Many of these phenotypes, which were characteristics of 35S::IPT transgenic plants, were observed in plants overexpressing KN1 gene, including increase in transformation rate, alteration of leaf morphology and loss of apical dominance. However, more flowers, shoots, roots and higher seed germination rate were observed in 35S::KN1 transgenic plants, compared to 35S::IPT transgenic plants. Higher cytokinin content was observed in 35S::IPT leaves than that in 35S::KN1 leaves.

Key words: IPT gene; KN1 gene; CaMV 35S; transgenic tobacco